水稻核不育突变体gsl5花粉壁单糖组分研究

摘要

Pollen wall plays vital role during microspore development and pollination in rice(Oryza Japonica). Abnormal pollen wall formation usually leads to male sterility. Callose wall,microspore primary wall development and sporonpollenin accumulation are key step to control pollen wall formation. Recent studies focus on callose wall formation during microspore development. Neither less,the knowledge about monosaccharide composition in the pollen wall is limited. In this study,we compared the monosaccharide composition in the pollen wall of the wide type rice with a male sterility mutant gsl5.%水稻花粉壁在花粉发育、适应环境和授粉识别过程中具有重要作用.花粉壁的发育异常会导致水稻雄性不育.在水稻花粉壁发育过程中,胼胝质层形成、初生外壁沉积和孢粉素的生物合成是关键步骤.目前对花粉初生外壁胼胝质层的研究较多,但对于水稻花粉壁的单糖组分缺乏整体性研究.我们利用花粉胼胝质层缺失的水稻突变体gsl5与野生型进行比较,对花粉壁单糖组分进行了色谱分析,初步研究了胼胝质层缺失对水稻花粉外壁组分的影响.