Screening of differentially expressed microRNA in ulcerative colitis related colorectal cancer

摘要

Objective:To investigate the differential expression of microRNA(miRNA) in colon between ulcerative colitis(UC) and ulcerative colitis related colorectal cancer(UCRCC).Methods:An UC mouse model was built by dextran sodium sulfate, and anUCRCC mouse model by dextran sodium sulfate and1,2-diformylhydrazine.RNAs were extracted from the colon, purified and hybridized with fluorescence-labeled miRNA oligonucleotide gene chip.Real-time fluorescence quantitativePCR was used to verify the expression variation of miRNA.SAM was employed for the data analysis.Results:The up-regulated miRNAs in colon cancer included has-miR-194, has-miR-215, has-miR-93, has-miR-192, has-miR-92a, has-miR-29b, and has-miR-20a(median false discovery rate<5%), while the down-regulated miRNAs were has-miR-1231, has-miR-195, has-miR-143, and has-miR-145(median false discovery rate<5%).Conclusions:Significant differential expression of miRNA was found between theUC mouse andUCRCC mouse, which may be related to the onset, erosion and transfer of colorectal cancer.