Effect of 5- AZn-2 '-deoxycytidine on proliferation of human lung adenocarcinoma cell line A549 in vitro

摘要

Objective:To explore effect of5-AZn-2 '-deoxycytidine on proliferation of human lung adenocarcinoma cell lineA549in vitro.Methods:Superoxide dismutase(SOD) activity was measured by hydroxylamine colorimetric method.Inhibition effect of5-AZn-2’ deoxycytidylic acid at different concentration and different time on growth ofA549 cell was determined byMTT assay.Methylene dioxyamphetamine(MDA) was measured by thiobarbituric acid colorimetric method.Effect of5-AZn-2’ deoxycytidylic acid on apoptosis ofA549 cell was determined by Hoechst33258 dyeing detection.Results:5-AZn-2' deoxycytidylic acid had significant inhibition effect on proliferation ofA549 cellsin vitro, and the inhibition was notably dependent on time and dosage during48-72 h;SOD level was significantly lower than those of control group(P<0.05, P<0.01),MDA level was significantly higher than those in the control group(P<0.05,P<0.01). A549 cells began to be in apoptosis after using5-AZn-2' deoxycytidylic acid.Conclusions:5-AZn-2' deoxycytidylic acid has significant inhibition effect on growth ofA549 cell, and can lead the change of lipid peroxidation.It indicates that the mechanism has relationship withA549 cell cycle tissue and induction factor of apoptosis.