Screening and Identification of Waterlogging Tolerance in Brassica napus Germplasm Resources

摘要

In order to evaluate,screen and identify waterlogging-tolerant Brassica napus resources and provide good germplasm materials for breeding of waterlogging-tolerant rape,608 B.napus germplasm materials from different sources were identified and screened for waterlogging-tolerant germplasms.The identification results showed that tested materials had dead seedling rates in the range of 0-100%,indicating that these rape germplasm materials varied extensively in waterlogging tolerance.Among the 608 materials,waterlogging-tolerant materials(grade I,relative dead seedling rate≤21%)accounted for 25.49%;non-tolerant materials(gradeⅣ+V,dead seedling rate≥61%)accounted for 29.43%;and other materials had waterlogging tolerance between the two.The identification results of waterlogging tolerance in the 349 rape germplasm resource from the upper,middle and lower reaches of Yangtze River and Huang-Huai Basin showed that materials from the lower Yangtze River had the lowest average dead seedling rate of 38.8%,which was remarkably lower than materials from other ecological regions,and waterlogging-tolerant materials among them accounted for 33.6%,which was also remarkably higher than materials from other ecological regions.It could thus be seen that materials from the lower Yangtze River have stronger waterlogging tolerance than those from other ecological regions overall.In this study,24 highly-tolerant B.napus germplasms(dead seedling rate<1%)were selected from the 608 B.napus germplasm materials,which could be used for breeding of waterlogging-tolerant rape and related fundamental research.