Isolation and Identification of Fusarium Wilt Pathogens from Bitter Gourd in Hainan Province

摘要

In order to isolate and identify the pathogens causing Fusarium wilt in bitter gourd in Hainan Province,China,four bitter gourd plants exhibiting symptoms of Fusarium wilt were collected from Tunchang County,Hainan Province.Four pathogen strains(HNTC-01,HNTC-02,HNTC-03 and HNTC-04)were isolated from the four plants,and identified via morphological observation and molecular techniques.The results showed that HNTC-01,HNTC-03 and HNTC-04 were F.oxysporum,while HNTC-02 was F.graminearum.The tests on pathogenicity and host specificity showed that HNTC-01,HNTC-03 and HNTC-04 were F.oxysporum Schl.f.sp.momordicae.Phylogenetic analysis revealed that HNTC-01,HNTC-03,HNTC-04 were clustered together with 14 strains of F.oxysporum,and HNTC-02 was clustered together with six strains of F.graminearum.In addition,rDNA-ITS region can be used to distinguish different Fusarium species,but can not be used to distinguish different biotypes within a Fusarium species.This study could provide a scientific basis for the diagnosis and prevention of wilt in bitter gourd.