提出和发展了基于毛细管–微球组合探针的任选区、高分辨显微成像新方法.建立了微球显微成像的物理模型,利用成像理论,推导出微球成像的放大倍率；采用3.0,4.4,5.6,7.5,10.0µm等不同直径的SiO2微球,对未经刻录的DVD光盘进行了微球显微成像实验,可以观察到DVD光盘的微纳米结构被明显放大且对比度显著提高,与理论计算结果相符合；采用毛细管微探针操纵微球的方法,实现了基于微球透镜阵列的样品微纳米结构的高分辨显微成像；在此基础上,进一步将毛细管微探针与微球组合,制备出毛细管–微球组合型探针,首次实现了基于微球透镜的样品任意区域高分辨显微成像.%In this paper, we propose a novel location-free high-resolution optical microscopic imaging method based on micropipette-microsphere combined probe. According to optical principle, a physical model is established to determine the value of magnification. With SiO2 microspheres of different diameters such as 3.0, 4.4, 5.6, 7.5 and 10.0 µm, microscopic imaging experiments are carried out. The results show that the microstructure of un-etched DVD disk can be significantly magnified and the image contrast is ob-viously improved, which is consistent well with the theoretical calculation. Microscopic imaging of microstructures based on SiO2 microsphere array is realized by controlling the microspheres with a micropipette. Furthermore, a kind of micropipette-microsphere combined probe is prepared, by which location-free high-resolution optical microscopic imaging has implemented for the first time.
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