Antibiotics induce prostagladin E2 production and cytotoxicity in equine chondrocytes that can be inhibited by avocado soybean unsaponifiables, glucosamine, and chondroitin sulfate.

摘要

Amikacin (AK) and enrofloxacin (EF) concentrations consistent with intra-articular and regional limb perfusion were evaluated for their effects on equine chondrocytes. We evaluated the production of prostaglandin E2 (PGE2) by equine chondrocytes in response to AK and EF administration, and if the combination of avocado soybean unsaponifiables (ASU), glucosamine (GLU), and chondroitin sulfate (CS) could reduce the production of PGE2. Monolayer cell cultures of equine chondrocytes were treated with clinically relevant concentrations of AK and EF plus combinations of ASU, GLU, and CS. AK and EF generated a dose dependent cytotoxicity. The induction of PGE2 following EF administration was significantly greater than PGE2 levels induced by positive controls. Induction of PGE 2 by EF was significantly reduced in chondrocytes pretreated with ASU, GLU, and CS. We have demonstrated for the first time that EF can induce production of PGE2 in equine chondrocytes and that this effect can be attenuated with the combination of ASU, GLU, and CS.