ASSEMBLY OF(SMALL ANIONS/LAYERED DOULE HYDROXIDES)n UTFs LUMINESCENT ULTRATHIN FILMS,ITS ENHANCED PHOTOLUMINESCENCE AND FLUORESCENT BIOSENSOR APPLICATION

目录

声明

ABSTRACT

摘要

Contents

Chapter 1 Introduction

1．1． Research Objective

1．2．Mechanism of fluorescence resonance energy transfer(FRET)in LDH based nanocomposites

1．3． Research Background

1．3．1．Two-dimensional nanomaterials

1．4．Two-dimensional LDHs thin films

1．4．1．Concept and Structure of LDHs

1．4．2．Preparation methods of LDH based nanocomposite ultrathin films

1．4．3．Fabrication and characterization of(LDH／CP)n UTF nanocomposites

1．4．4．Driving forces and guests of the LDH based assembly films

1．5． Optical and optoelectric properties of (LDH／CP)n nanocomposites

1．5．1．Optical Properties

1．6．Principles for the luminescence performance of LDH based nanocomposites and their application as fluorescent biosensor

1．6．1．LDH based fluorescent sensor used for identification of VOC solvents

1．6．2．LDH based fluorescence sensor used for the identification of Biomolecules

1．6．3．LDH based nuorescence sensor used for the identification of ssDNA

1．7．Current research status of LDH based biological inorganic fluorescent sensor Fluorescence biosensor

1．8． Content，objective and significance of this dissertation

Chapter 2 Assembly of(LY／LDH)n UTF with enhanced cyan luminescence and biosensor application

2．1． Introduction

2．2． Experiments

2．2．1．Raw materials

2．2．2．Characterization

2．2．3．Assembly of the small anion 2D cascade forster resonance energy transfer(FRET)luminescent(LY／LDH)n UTFs

2．3． Results and Discussion

2．3．1．Characterization of the Small anion 2D cascade forster resonance energy transfer(FRET)luminescent(LY／LDH)n UTFs

2．3．2．Morphology Characterization of the(LY／LDH)n UTFs

2．3．3．Effect of LDHs on luminescent properties of LY

2．3．4． Fluorescence enhancement effect of LDH on LY films

2．3．5．Analysis of fluorescence enhancement of immune globulin G(IgG)by(LY／LDH)n UTFs

2．3．6．Reversible fluorescence response for the immune globulin G(IgG)molecule

2．4．Conclusion

Chapter-03 Assem bly of(CdTe QDs@SF／LDH)n UTFs enhanced photoluminescence and biosensor application

3．1．Introduction

3．2． Experiments

3．2．1．Raw materials

3．2．2．Characterization

3．2．3．Purification of silk fibroin(SF)

3．2．4．Synthesis of CdTe QDs

3．2．5．Fabrication of(CdTe QDs／LDH)n and(CdTe QDs@SF／LDH)n UTFs

3．3．1．Fabrication of the(CdTe QDs@SF／LDH)n UTFs

3．3．2．Morphology Structure elucidation of the(CdTe QD@SF／LDH)n UTFs

3．3．3．Effect of SF on luminescent properties of CdTe QDs

3．3．4．Fluorescence enhancement effect of SF on CdTe QDs in solution state

3．3．5．Fluorescence enhancementeffect of Silk Fibroin(SF)on CdTe QDs in ultrathin films

3．3．6． Fluorescence response of the(CdTe QDs@SF／LDH)n UTFs for immune globulinG(Ig G)

3．3．7． Effect of LDHs on the stability of(CdTe QD@SF／LDH)30 UTF

3．4．Conclusion

Chapter-04 Assembly of(CdTe QDs@GAG／LDH)n UTFs enhanced photoluminescence and biosensor application

4．1． Introduction

4．2． Experiments

4．2．2．Characterization

4．2．3．Synthesis of CdTe QDs

4．2．4．Synthesis of the LDH colloidal suspension

4．2．6．Preparation of(CdTeQDs@GAG／LDH)n UTFs

4．3． Results and discussion

4．3．1．Fabrication of(CdTe QDs@GAG／LDH)n UTFs

4．3．2．Qualitative and quantitative analysis of water molecules using LDH-based fluorescence sensor，(CdTe QDs@GAG／LDH)n UTFs ultrathin films

4．3．3．Qualitative and quantitative analysis of k+ and Na+ ions using (CdTe QDs@GAG／LDH)n UTFs ultrathin films using fluorescence spectroscopy

4．4． Conclusion

Chapter 5 Fabrication of(ssDNA@DAPI／LDH)n UTFs and biosensor application

5．1． Introduction

5．2． Experimental section

5．2．1．Materials and apparatus

5．2．2．Assembly of the 2-D cascade foster resonance energy transfer(FRET)luminescent ultrathin films(ssDNA@DAPI／LDH)n UTFs

5．3． Results and discussion

5．3．1．Fabrication and Characterization of the 2D cascade forster resonance energy transfer(FRET)Luminescent(ssDNA@DAPI／LDH)n UTFs ultrathin films

3．2． Morphology and Structure Charaeterization of the(ssDNA@DAPI／LDH)n UTFs ultrathin films

5．3．3．Selective fluorescence response for the conventional complementary single stranded DNA(ssDNA)sequence

5．3．4．Selective fluorescence response for different telomere sequence

5．3．5．Reversible fluorescence response for the long complementary ssDNA fragments

5．4． Conclusion

Chapter 6 Fabrication of(Black Pn／LDH)n UTFs enhanced photoluminescence

6．1． Introduction

6．2． Experiments

6．2．1．Raw materials

6．2．2．Characterization

6．2．3．Assembly of the small anion luminescent(BPN／LDH)n UTFs

6．3． Results and Discussion

6．3．1．Characterization of the Small anion luminescent(BPN／LDH)n UTFs

6．3．2．Morphology Characterization of the(BPN／LDH)n UTFs

6．4．Conclusion

Thesis Conclusion

References

List of Publications

Acknowledgement

Introduction to author

Introduction to supervisor