Preparation of a Single Laryer Enzyme Electrode by Plasma Polymerisation Technique

摘要

A biosensor can be defined as a device which responds to the presence of a specific analyte (a chemical substance that needs to be measured) by producing an electrical signal proportional to the concentration of the analyte. It is usually constructed from three components: receptor, transducer and electronics (amplification and display). The receptor is composed of immobilised, biologically sensitive material (biocatalyst). molecular recognition f a specific sample analyte by the respective receptor is realized by the lock-key principle. Enzymes,proteins, antibodies, DNA. organelles, microbial cells or whole sections of mammalian or plant tissues may constitte the tiological recognition system. This element may be protectd by a membran which is permeable to certain moblecules in the environment. The most important characteristic about the biocatalyst, other than the ability to detect a specific compound, is its role in the transmittance of an energy impulse proportional to the reaction of the biocatalyst with the measured analyte. Thus, the receptor must have intimate contact with a suitable transducing system. The transducer is an electrical device which converts the effects of the biocatalytical reaction into forms which can be electronically amplified, stored and displayed (1).