Label-Free Imaging of Live Cell Using Large-Scale Photonic Crystal Nanolaser Array

摘要

We integrated 441 photonic crystal nanolasers and applied it to label-free live cell imaging. Since the mechanical strength of the nanolaser array was improved by bonding on a glass substrate, the imaging area was expanded from previous 25 × 25 μm~2 to 100 × 100 ~2. We successfully acquired cell images, which should reflect the intracellular and/or the attachment conditions. By continuously mapping the wavelengths of all nanolasers, we successfully observed the time-dependent images displaying the cell behaviors. Reagents were injected to stimulate the cells, and the observation was continued until the cell reaction was saturated. The results show the reasonable behaviors against the reagents.