Characterization and determination of Acetyl-coenzyme A carboxylase (ACCase) from Eukaryotic Alga Chlorella sp. USTB-01

机译：真核生物藻藻藻酸乙酰酶羧基酶（Accase）的表征与测定。 USTB-01.

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In the present study, the Acetyl-coenzyme A carboxylase (ACCase) of Chlorella sp. USTB-01 purified and characterized. Molecular weight of ACCase was calculated to be about 456 kD and was comprised of two identical subunits of 229 kD. The enzymatic characteristics isoelectric point was 6.7. Moreover, a rapid and sensitive assay of High performance liquid chromatography (HPLC) was established to measure overall activity of ACCase in low protein content condition by detect the ACCase activity by measuring ADP production and Acetyl-coenzyme A (Acetyl-CoA).

机译：在本研究中，乙酰辅酶甲基辅酶A SP的羧基酶（Accase）。 USTB-01纯化和特征。丙酸分子量计算为约456kd，并由两个相同的229kd亚基组成。酶特性等电点为6.7。此外，建立了高效液相色谱（HPLC）的快速和敏感的测定，以通过测量ADP生产和乙酰辅酶A（乙酰-COA）来测量低蛋白质含量条件下的Accase的总体活性。

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《International Conference on Advances in Energy and Environmental Science》|2013年||共5页
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Haiou Wang; Yang Jing; Zhe Tang;
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中图分类 TB3-532;
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Acetyl-coenzyme A carboxylase; characterization; determination; electrophoresis; High performance liquid chromatography;

机译：乙酰辅酶A羧化酶;表征;测定;电泳;高效液相色谱;

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3. The toxicological effects of oxybenzone, an active ingredient in suncream personal care products, on prokaryotic alga Arthrospira sp. and eukaryotic alga Chlorella sp. [J] . Zhong Xin, Downs Craig A., Che Xingkai, Aquatic Toxicology . 2019,第期

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4. Characterization and determination of Acetyl-coenzyme A carboxylase (ACCase) from Eukaryotic Alga Chlorella sp. USTB-01 [C] . Haiou Wang, Yang Jing, Zhe Tang International Conference on Advances in Energy and Environmental Science . 2013

机译：真核生物藻藻藻酸乙酰酶羧基酶（Accase）的表征与测定。 USTB-01.
5. Characterization of the carboxyltransferase domain in acyl -CoA carboxylase (ACCase) from Streptomyces coelicolor and Mycobacterium tuberculosis. [D] . Lin, Ting-Wan. 2006

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6. Biochemical and molecular biological characterization of CAC2 the Arabidopsis thaliana gene coding for the biotin carboxylase subunit of the plastidic acetyl-coenzyme A carboxylase. [O] . J Sun, J Ke, J L Johnson, 1997

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7. Multiple resistance to acetolactate synthase (ALS)- and acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicides in black-grass (Alopecurus myosuroides Huds.) populations from Poland [O] . Adamczewski Kazimierz, Kierzek Roman, Matysiak Kinga 2016

机译：来自波兰的黑草（alopecurus myosuroides Huds。）种群中对乙酰乳酸合成酶（aLs）和乙酰辅酶a羧化酶（aCCase）的抑制性除草剂的多重抗性

Characterization and determination of Acetyl-coenzyme A carboxylase (ACCase) from Eukaryotic Alga Chlorella sp. USTB-01

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