Determination of Chlorophyll in Water by Fluorophotometry with Chlorophyll-a Extraction from Microcystis Aeruginosa Instead of Chlorophyll Standard substance

摘要

Microcystis aeruginosa is common in freshwater lakes, and developed easy. Instead of chlorophyll standard substance, fresh chlorophyll extraction from Microcystis aeruginosa applied to the determination of chlorophyll-a in water by fluorophotometry. Thus the relationship between concentration of chlorophyll-a and fluorescence intensity of Microcystis aeruginosa in water was established. The fluorescence intensity responded with the concentration of chlorophyll-a extract linearly in the range of 30-1800 ug/L with a correlative coefficient of 0.9947. Comparision was made between chlorophyll standard and the chlorophyll-a extraction of Microcystis aeruginosa, and no significant difference was found. In certain range of pH value and time, chlorophyll-a extraction from Microcystis aeruginosa is stable, and can be used to reflect the growth of algae in water quick. conveniently and accurately.