The saponin content in white sugar and thick juice from Polish sugar factories was determined by a method based on SPE followed by HPLC. The content of saponins expressed as oleanolic acid monoglucuronide in white sugar from Polish factories amountedto 0.1-0.4 mg of oleanolic acid monoglucuronide in 1 kg of white sugar and the mean value was 0.25 mg of oleanolic acid monoglucuronide in 1 kg of white sugar. The content of saponins in thick juice expressed as oleanolic acid monoglucuronide in thick juices from Polish sugar factories amounted to 100-135 mg of oleanolic acid monoglucuronide in 1 kg of dry substance of thick juices. The mean value was 120 mg of oleanolic acid monoglucuronide in 1 kg of dry substance of thick juices. The free oleanolic acid monoglucuronide was detected in every sample of thick juices. The content of free oleanolic acid monoglucuronide amounted to 3.5- 40 mg of oleanolic acid monoglucuronide in 1 kg of dry substance of thick juices and the mean value was 13 mg oleanolicacid monoglucuronide in 1 kg of dry substance of oleanolic acid monoglucuronide.
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