Screening, Isolation, and Identification of Bacillus coagulans C2 in Pu'er Tea

摘要

Pu'er Tea is a popular post-fermented tea and has a variety of health benefits. Many microorganisms, especially lactobacillus, exist and play an important role in the fermentation process of Pu'er tea. In this study, we try to screen some lactobacillus by plate method from the Pu'er tea, and strain C2 with significant cycle isolated from Pu'er tea. 16S rRNA gene sequencing results showed that strain C2 had 99 % sequence similarity to that of the type strain of three Bacillus coagulans (NR 102791.1, CP002472.1, NR 115727.1), and the results show that methyl red test and V&P test were positive. Sugar fermentation test suggested that strain C2 produced acid but not gas, which is identical to B. coagulans. This study confirmed that lactic acid bacteria play a role in Pu'er tea fermentation.