Fluorescence-based tissue biopsy needle: in vivo optical diagnosis in rat model with low cost arc-lamp-based system

摘要

Abstract: gnostic capability of an optical biopsy probe combined with a tumor selective fluorescence drug (Photofrin II$+R$/) was tested in an in vivo animal model. The optical biopsy system is comprised of an arc lamp, an optical contact probe, and low cost detectors. We illuminated tissue with blue (405 nm) light and detected the diffusely reflected excitation light, the tissue autofluorescence (500 - 540 nm), and the drug fluorescence near 630 nm. By using the appropriate signal ratios, normal, inflamed and malignant lymph nodes could be distinguished with a high specificity and sensitivity (85 - 95%). !20