Fruit Body Development and Genetic Recombination of SOmatic Hybrids by protoplast Fusion in Basidiomycete Pleurotus

摘要

Fusion products between compatible and incompatible species were obtained by protoplast fusion. The somatic hybrids of inter-compatible spectied formed heterokaryons, while somatic hybrids of inter-incompatible species formed synkaryons that retained genes from both parents. Fruit body development of synkaryons was influenced by several factors including light, temperature, nutritions, and physical states fo the culture media. Clamp connections were present in most of mature fruit bodies though initial fusion colonies were lack. The synkaryons showed unique patterns compared with heterokaryons such as slow growth rate, clamp connection formation at mitosis, and asynchronous fruiting body. Four possible genotypes that were protorophs, auxotroph of one parental type, auzotroph of th other parental type, and auxotrophicrecombinants can be generated from progenies of somatic hybrids. Some synkaryons recovered non-expected alleles, indicating that macrogneome eas interated into the random site of receipient chromosomes after genome ressortment.