Ray-Traced Monte Carlo simulation tool for computer-aided design of tissue fluorescence sensing system

摘要

In designing fluorescence spectroscopy and imaging systems for biological applications, a sample's own optical properties can significantly affect system performance. The independent use of Monte Carlo codes or optical design software often does not provide complete and accurate modeling of the fluorescence excitation and emission path through the optical components and turbid sample. We have developed a ray-traced Monte Carlo (RTMC) simulation method to track the complete light path by combining MC codes and ray-tracing software. Computational and experimental verification results demonstrate that RTMC has the potential to become a useful tool for designing tissue fluorescence sensing systems.