Lens-less holographic microscope with large visual depth

摘要

A new method of the lens-less holographic microscope is proposed for recording a high-resolution 3-D image with large visual depth. The light beam with a large viewing angle diffracted from the object surface is recorded by illuminating the three-dimentional object with a large incidence angle. A number of partial holograms are sequentially recorded with one CCD located at the fixed position by changing an incident angle of illumination light. These partial holograms are arranged in order to generate a hologram with large viewing-zone angle for the reconstruction of a high-resolution image. Optical experiments are carried out to demonstrate the present new method of the holographic microscope. Results show that a high-resolution image is reconstructed from the wide hologram generated from a number of partial holograms and the resolving power of reconstructed image is improved as the number of partial holograms becomes large.