A three-dimensional imaging method for biological samples under liquid

摘要

This paper presents a new non-contact method for measuring 3-D features of biological materials. The method can provide 3-D images of samples under liquid such as water with a microscopic resolution. One of the advantages of measuring under liquid includes protecting air sensitive surfaces of biological materials. The method is based on optical microscopy with machine vision and precision actuation. The geometric information of 3-D samples can be obtained by analyzing the focus errors of the image pixels. Experimental results have been presented and discussed to demonstrate the technique.