Multi-photon micro-spectroscopy of biological specimens

摘要

The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multiphoton fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.