Two-photon Fluorescence Imaging of Rat Aorta

摘要

Two-photon excitation fluorescence microscopy has become increasingly popular in 3D fluorescence imaging of tissue samples. Observation depth is an important indicator for the performance of the technique. Using the principle of tissue optics, we analyzed quantitatively the effects of optical properties, such as abosrption coefficient, scattering coefficient, and the scattering anisotropy factor of the tissue, to the observation depth. An analytical relation between the fluorescence and the observation depth has been derived and tested against experimental results for rat aorta elastic lamina.