The Study of Insoluble Components in Human Gallstones by Raman and IR Spectroscopy

摘要

Human gallstones were immersed with chloroform, ethanol and ether. The insoluble components were studied by normal Raman and surface-enhanced Raman (SER) and FT-infrared (IR) spectroscopy. The results show that bilirubinate salts and proteins are the main components in the insoluble materials of gallstones. The secondary structure of the proteins in gallstones are dominated by alpha-helix structure. The results suggest that proteins play a key role in the formation of gallstones.