Impact of nanomaterials on in vitro and in vivo systems: role of nanoscale features in nanotoxicology

摘要

The interactions between biological systems and nanostructured materials are attracting great interest, due to the possibility to open up novel concepts for the design of smart nano-biomaterials that actively play a functional biological role. On the other hand, the assessment of the potential toxic effects arising from such interactions is gaining increasing attention, and a new field known as nanotoxicology is strongly emerging. In this frame, we investigated the response of human neurons to gold surfaces with different levels of nanoroughness, finding out that neurons are capable to sense and actively respond to these nanotopography features. These nanostructured substrates were also investigated to explore the impact of nanotopography on morphology and genomics of adherent bacteria. A multidisciplinary approach was exploited to characterize bacteria-nanostructured surface interactions, observing that type-1 fimbriae disappear in bacteria grown onto nanorough substrates. We also show how nanoparticles interact with biomolecules in culture media and in vitro and in vivo biological systems, by investigating the toxic effects of a wide range of nanomaterials (AuNPs, QDs, SiO_2 NPs), demonstrating the key role of size, shape, and surface coating.