A pair of degenerate primers was designed according to the conservative regions at the 3'' terminus of the floral meristem identity genes (LEAFY). A 361 bp cDNA fragment of LEAFY homologous gene (PcBLFY) coding for 120 amino acid residues was amplified via RT-PCR from flower buds of male, female and hermaphrodite plant of Pistacia chinensis Bunge respectively. Furthermore, 989 bp genomic fragment of PcBLFY was isolated by homology cloning strategy. Sequence analysis indicated that this fragment contained a 628 bp intron and the splicing sites were similar to other LEAFY homologous genes. The deduced amino acid sequence from the exons was blast in GenBank, it was found that there was 89%~99% similarity between PcBLFY and the corresponding regions of LEAFY and its orthologues. The three sexual plants shared a same PcBLFY gene fragment.
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机译：根据花分生组织同一性基因（LEAFY）3''末端的保守区域设计了一对简并引物。通过RT-PCR分别从黄连木的雄性，雌性和雌雄同体植物的花蕾中扩增了一个LEAFY同源基因（PcBLFY）的361 bp cDNA片段，该片段编码120个氨基酸残基。此外，通过同源克隆策略分离了PcBLFY的989bp基因组片段。序列分析表明该片段含有一个628 bp的内含子，其剪接位点与其他LEAFY同源基因相似。从外显子推导的氨基酸序列在GenBank中被发现，发现PcBLFY与LEAFY及其直系同源物的相应区域有89％〜99％的相似性。这三种有性植物共享相同的PcBLFY基因片段。
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