Seed Conservation under –196 ℃ in Liquid Nitrogen. The Use of Paper Tubes for Seed Cryopreservation.

摘要

The effects of storage at the temperature of liquid nitrogen (LN, –196 ℃) on the viability of Allium ceparnseeds was investigated using sealed paper tubes for seed storage at low temperatures. Seed germination wasrnstudied after its storage in LN_2 during 2 and 4 weeks. The final germination percentage of seed samples afterrncryopreservation yielded similar results and did not show significant differences in growth and developmentrnof seedlings. This study demonstrates that seed with low moisture content packing in paper could be used forrnseed preservation in LN_2.