Integrated liquid chromatography-solid phase extraction-nuclear magnetic resonance/mass spectrometry (LC-SPE-NMR/MS) is powerful platform,which was used to analyze the target components in complex system,for instance,bioactive compounds in traditional Chinese medicines,biomarkers in animal biofluids,toxic components in food or environmental samples.In this study,we used this platform to comprehensively screen all of the flavonoids in rhizoma smilacis glabrae based a self-made database using high resolution MS and MS/MS of ultra-high performance liquid chromatography-quadrupole-time of flight/mass spectrometry (UHPLC-Q-TOF/MS),we found over thirty potential new flavonoids,further qualified using LC-SPE-NMR,among of them,six compounds were identified as new flavonoids by bath of 1Dand 2D NMR spectra.
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